A sample of pure Terbium-161 with radioactivity over 5 GBq was prepared at the Faculty of Nuclear Sciences and Physical Engineering, CTU in Prague, in the same way as the sample in 1 GBq milestone. Total amount of Tb-161 obtained after separation was 7386,7 MBq (related to the end of beam). Radionuclide purity of prepared sample reached 99,9996%.